師資
田瑞軍博士，南方科技大學(xué)化學(xué)系講席教授、國家杰出青年科學(xué)基金獲得者、化學(xué)生物學(xué)與組學(xué)分析研究中心主任、南方科技大學(xué)技術(shù)轉(zhuǎn)移中心主任、南方科技大學(xué)光明高等研究院副院長、南方科技大學(xué)第一附屬醫(yī)院兼職教授；中國蛋白質(zhì)組組織CNHUPO副理事長、中國化學(xué)會色譜專業(yè)委員會副主任委員、中國質(zhì)譜學(xué)會理事和中國分子系統(tǒng)生物學(xué)學(xué)會理事。2008年在中國科學(xué)院大連化學(xué)物理研究所獲得分析化學(xué)博士學(xué)位。先后在加拿大渥太華大學(xué)Ottawa Institute of Systems Biology和多倫多大學(xué)/西奈山醫(yī)院Lunenfeld-Tanenbaum Research Institute完成博士后研究。2014年起受聘南方科技大學(xué)化學(xué)系，致力于蛋白質(zhì)組學(xué)的方法學(xué)和技術(shù)研究，并強調(diào)其在細胞信號轉(zhuǎn)導(dǎo)和胰腺癌腫瘤微環(huán)境等生物醫(yī)學(xué)研究方向的應(yīng)用。已在國內(nèi)外主流學(xué)術(shù)期刊Nature、Nat. Chem. Biol.、Cell Chem. Biol.、PNAS、Nat. Commun.、Mol. Cell. Proteomics、Anal. Chem.等上發(fā)表論文百余篇。曾榮獲由國際蛋白質(zhì)結(jié)構(gòu)分析和蛋白質(zhì)組學(xué)協(xié)會（IAPSAP）頒發(fā)的2012 Young Investigator Award、廣東省杰出青年基金和深圳市鵬城學(xué)者特聘教授等。主持國家科技部重點研發(fā)計劃、國家自然科學(xué)基金委重大研究計劃集成項目、深圳市功能蛋白質(zhì)組學(xué)重點實驗室等項目。曾擔(dān)任中國化學(xué)會第23屆全國色譜學(xué)術(shù)報告會共同執(zhí)行主席、第5屆中加系統(tǒng)生物學(xué)研討會和第2屆中國蛋白質(zhì)組組織（CNHUPO）青年學(xué)者研討會共同主席等。擔(dān)任分析化學(xué)、色譜雜志編委和Curr. Opin. Chem. Biol. Guest Editor。
代表性承擔(dān)項目：
1. 國家科技部-國家重點研發(fā)計劃，組織微環(huán)境蛋白質(zhì)組可視化的前沿技術(shù)，2024.12-2029.11，項目負責(zé)人
2. 國家自然科學(xué)基金-重大研究計劃，腫瘤微環(huán)境中關(guān)鍵蛋白質(zhì)復(fù)合物動態(tài)修飾的多維度發(fā)現(xiàn)與表征，2023.01-2025.12，項目負責(zé)人
3. 國家自然科學(xué)基金-杰出青年科學(xué)基金項目，22125403，復(fù)雜生物樣品分離與質(zhì)譜分析，2022.01-2026.12，項目負責(zé)人
4. 深圳市功能蛋白質(zhì)組學(xué)重點實驗室，2024.02-2026.02，項目負責(zé)人
6. 深圳市科創(chuàng)局-重點項目，基于多組學(xué)的腫瘤耐藥新靶點篩選及分子機制研究，2025.03-2028.03，項目負責(zé)人
代表性論文：
Invited reviews:
1. P. W. Huang,? W. N. Gao,? C. Y. Fu,? R. J. Tian,* Functional and clinical proteomic exploration of pancreatic cancer, Mol. Cell. Proteomics, 2023, 22, 100575. (Invited Reviews)
2. Z. Y. Liu,? X. Chen,? S. R. Yang, R. J. Tian,* F. J. Wang,* Integrated mass spectrometry strategy for functional protein complex discovery and structural characterization, Curr. Opin. Chem. Biol., 2023, 74, 102305. (Invited Reviews)
3. Y. H. Mao, X. Wang, P. W. Huang, R. J. Tian,* Spatial proteomics for understanding the tissue microenvironment, Analyst, 2021, 146, 3777-3798. (Invited Reviews)
4. J. N. Zheng,? X. Chen,? Y. Yang, C. S. H. Tan, R. J. Tian,* Mass spectrometry-based protein complex profiling in time and space, Anal. Chem., 2021, 93, 598–619. (Invited Reviews)
5. X. T. Ye,? J. Tang,? Y. H. Mao,? X. Lu, Y. Yang, W. D. Chen, X. Y. Zhang, R. L. Xu, R. J. Tian,* Integrated proteomics sample preparation and fractionation: method development and applications, TrAC Trends Anal. Chem., 2019, 120, 115667. (Highlighted as Front Cover)
6. R. J. Tian,* Exploring intercellular signaling by proteomic approaches, Proteomics, 2014, 14, 498-512. (Invited Reviews)
Research articles:
1. Y. H. Mao,? Y. Li,? Z. D. Zheng,? Y. F. Xu,? M. Ke, A. He, F. C. Liang, K. R. Zhang, X. Wang, W. N. Gao, R. J. Tian,* All-at-once spatial proteome profiling of complex tissue context with single-cell-type resolution by proximity proteomics, Cell Systems, 2025 May 5:101291.
2. P. W. Huang,? W. N. Gao,? C. Y. Fu,? M. Wang,? Y. G. Li,? B. Z. Chu, A. He, Y. Li, X. M. Deng, Y. H. Zhang, Q. Kong, J. X. Yuan, H. B. Wang, Y. Shi,* D. Gao,* R. Y. Qin,* T. Hunter, R. J. Tian,* Clinical functional proteomics of intercellular signaling in pancreatic cancer, Nature, 2025, 637, 726–735.
3.Y. F. Xu,? X. Wang,? Y. Li,? Y. H. Mao,? Y. R. Su, Y. Z. Mao, Y. Yang, W. N. Gao, C. Y. Fu, W. D. Chen, X. T. Ye, F. C. Liang, P. Z. Bai, Y. Sun, S. P. Li, R. L. Xu, R. J. Tian,* Multimodal single cell-resolved spatial proteomics reveal pancreatic tumor heterogeneity, Nat. Commun., 2024, 15, 10100.
4. Y. H. Mao,? Y. Chen,? Y. Li,? L. D. Ma,? X. Wang, Q. Wang, A. He, X. Liu, T. Y. Dong, W. N. Gao, Y. F. Xu, L. Liu, L. Ren, Q. Liu, P. Zhou, B. Hu, Y. W. Zhou,* R. J. Tian,* Z. L. Shi,* Deep spatial proteomics reveals region-specific features of severe COVID-19-related pulmonary injury, Cell Reports, 2024, 43, 2,113689.
5. Q. Wu,? J. N. Zheng,? X. T. Sui,? C. Y. Fu,? X. Z. Cui, B. Liao, H. C. Ji, Y. Luo, A. He, X. Lu, C. S. H. Tan,* R. J. Tian,* High-throughput drug target discovery by fully automated proteomics sample preparation platform, Chemical Science, 2024, 15, 2833-2847.
6.Y. C. Weng,? W. D. Chen,? Q. Kong,? R. X. Wang, R. X. Zeng, A. He, Y. J. Liu, Y. H. Mao, Y. Q. Qin, W. S. C. Ngai, H. Zhang, M. Ke, J. Wnag, R. J. Tian,* P. R. Chen,* DeKinomics pulse-chases kinase functions in living cells, Nat. Chem. Biol., 2024, 20, 615-623.
7. J. N. Zheng,?* Z. D. Zheng,? C. Y. Fu,? Y. C. Weng, A. He, X. T. Ye, W. N. Gao, R. J. Tian,* Deciphering intercellular signaling complexes by interaction-guided chemical proteomics, Nat. Commun., 2023, 14, 4138.
8. X. Chen,? S. P. Ji,? Z. Y. Liu, X. Yuan, C. S. Xu, R. X. Qi, A. He, H. Zhao, H. P. Song, C. L. Xiao, W. N. Gao, P. Chen, R. Luo, P. F. Li,* F. J. Wang,* X. M. Yang, R. J. Tian,* Motif-dependent immune co-receptor interactome profiling by photoaffinity chemical proteomics, Cell Chem. Biol., 2022, 29, 1024-1036.
9. M. Ke,? X. Yuan,? A. He, P.Y. Yu, W.D. Chen, Y. Shi, T. Hunter, P. Zou, R. J. Tian,* Spatiotemporal Profiling of Cytosolic Signaling Complexes in Living Cells by Selective Proximity Proteomics, Nat. Commun., 2021, 12, 71.
10. Y. Shi,* W. N. Gao, N. K. Lytle, P. W. Huang, X. Yuan, A. M. Dann, M. Ridinger, K. E. DelGiorno, G. Erikson, H. Y. Sun, J. Meisenhelder, E. Terenziani, P. Santisakultarm, U. Manor, R. L. Xu, M. Leblanc, S. E. Umetsu, E. A. Collisson, A. M. Lowy, T. Reya, T. R. Donahue, M. Downes, G. M. Wahl, R. M. Evans, T. Pawson, R. J. Tian,* T. Hunter,* Targeting LIF-mediated paracrine interaction for pancreatic cancer therapy and monitoring, Nature, 2019, 569, 131-135.
11. B. Z. Chu,? A. He,? Y. T. Tian, W. He, P. Z. Chen, J. T. Hu, R. L. Xu, W. B. Zhou, M. J. Zhang, P. Y. Yang, S. S. C. Li, Y. Sun,* P. F. Li,* T. Hunter, R. J. Tian,* Photoaffinity-engineered protein scaffold for systematically exploring Native phosphotyrosine signaling complexes in tumor samples. Proc. Natl. Acad. Sci. U. S. A., 2018, 115, E8863-E8872.
12. R. J. Tian,*? H. P. Wang,? G. D. Gish, E. Petsalaki, A. Pasculescu, Y. Shi, M. Mollenauer, R. D. Bagshaw, N. Yosef, T. Hunter, A. Gingras, A. Weiss,* T. Pawson, A combinatorial proteomic analysis of intercellular signaling applied to the CD28 T cell co-stimulatory receptor, Proc. Natl. Acad. Sci. U. S. A. , 2015, 112, E1594–E1603.
13. Q. Kong,? M. Ke,? Y. C. Weng, Y. Q. Qin, A. He, P. F. Li,* Z. W. Cai,* R. J. Tian,* Dynamic phosphotyrosine-dependent signaling profiling in living cells by two-dimensional proximity proteomics, J. Proteome Res., 2022, 21, 2727–2735.
14. Q. Kong,? Y. C. Weng,? Z. D. Zheng, W. D. Chen, P. F. Li,* Z. W. Cai,* R. J. Tian,* Integrated and high-throughput approach for sensitive analysis of tyrosine phosphoproteome, Anal. Chem., 2022, 94, 40, 13728–13736.
15. Y. H. Mao,? P. Z. Chen,? M. Ke, X. Chen, S. P. Ji, W. D. Chen, R. J. Tian,* Fully integrated and multiplexed sample preparation technology for sensitive interactome profiling, Anal. Chem., 2021, 93, 5, 3026–3034.
16. P. W. Huang, Q. Kong, W. N. Gao, B. Z. Chu, H. Li, Y. H. Mao, Z. W. Cai, R. L. Xu, R. J. Tian,* Spatial proteome profiling by immunohistochemistry-based laser capture microdissection and data-independent acquisition proteomics, Anal. Chim. Acta, 2020, 1127, 140-148.
17. Q. Kong,? P. W. Huang,? B. Z. Chu, M. Ke, W. D. Chen, Z. D. Zheng, S. P. Ji, Z. W. Cai, P. F. Li, and R. J. Tian,* High-throughput and integrated chemical proteomic approach for profiling phosphotyrosine signaling complexes, Anal. Chem., 2020, 92, 8933-8942.
18. X. Lu,? Z. K. Wang,? Y. Gao, W. D. Chen, L. J. Wang, P. W. Huang, W. N. Gao, M. Ke, A. He, and R. J. Tian,* Autoproteome chip system for fully automated and integrated proteomics sample preparation and peptide fractionation, Anal. Chem., 2020, 92, 8893-8900.
19. P. W. Huang, H. J. Li, W. N. Gao, Z. W. Cai,* R. J. Tian,* A fully integrated spintip-based approach for sensitive and quantitative profiling of region-resolved in vivo brain glycoproteome, Anal. Chem., 2019, 91, 9181-9189.
20. R. L. Xu,? J. Tang,? Q. T. Deng, W. He, X. J. Sun, L. Xia, Z. Q. Cheng, L. S. He, S. Y. You, J. T. Hu, Y. X. Fu, J. Zhu, Y. X. Chen, W. N. Gao, A. He, Z. Y. Guo, L. Lin, H. Li, C. F. Hu, R. J. Tian,* Spatial-resolution cell type proteome profiling of cancer tissue by fully integrated proteomics technology, Anal. Chem., 2018, 90, 5879?5886.
21. W. D. Chen, S. Wang, S. Adhikari, Z. H. Deng, L. J. Wang, L. Chen, M. Ke, P. Y. Yang, R. J. Tian,* Simple and integrated spintip-based technology applied for deep proteome profiling, Anal. Chem., 2016, 88, 4864-4871.